Characterization of IND-6, a Highly Divergent IND-type Metallo-β-lactamase from Chryseobacterium indologenes strain 597 isolated in Burkina Faso
نویسندگان
چکیده
*Corresponding author; mailing address: Jean-Denis Docquier, Dipartimento di Biologia Molecolare, Università di Siena, Policlinico “Le Scotte”, I-53100 Siena, Italy. Tel. 39 0577 233134; Fax: 39 0577 233870; e-mail: [email protected]. Copyright © 2009, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved. Antimicrob. Agents Chemother. doi:10.1128/AAC.01607-08 AAC Accepts, published online ahead of print on 3 August 2009
منابع مشابه
Identification and characterization of a new metallo-beta-lactamase, IND-5, from a clinical isolate of Chryseobacterium indologenes.
A new natural IND-type metallo-beta-lactamase variant, IND-5, was identified in a clinical isolate of Chryseobacterium indologenes. IND-5 shared 92.8% and 92.4% amino acid homology with IND-1 and IND-3, respectively. Purified enzyme (pI = 8.8, M(r) = 25,000) was able to hydrolyze penicillins, some narrow- and expanded-spectrum cephalosporins, and carbapenems but not monobactams.
متن کاملCharacterization of CIA-1, an Ambler class A extended-spectrum β-lactamase from Chryseobacterium indologenes.
An Ambler class A β-lactamase gene, bla(CIA-1), was cloned from the reference strain Chryseobacterium indologenes ATCC 29897 and expressed in Escherichia coli BL21. The bla(CIA-1) gene encodes a novel extended-spectrum β-lactamase (ESBL) that shared 68% and 60% identities with the CGA-1 and CME-1 β-lactamases, respectively. bla(CIA-1)-like genes were detected from clinical isolates. In addition...
متن کاملGenetic diversity of carbapenem-hydrolyzing metallo-beta-lactamases from Chryseobacterium (Flavobacterium) indologenes.
The class B carbapenem-hydrolyzing beta-lactamase IND-1 has been characterized for Chryseobacterium indologenes strain 001. With internal primers for the bla gene for IND-1 (bla(IND-1)) and an internal bla(IND-1) probe, PCR amplifications failed, while hybridization results were positive when DNA from another C. indologenes isolate, strain CIP101026, was used as a template. Thus, a bla(IND)-rel...
متن کاملIdentification and Characterization of a New Metallo- -Lactamase, IND-5, from a Clinical Isolate of Chryseobacterium indologenes
Identification and Characterization of a New Metallo-Lactamase, IND-5, from a Clinical Isolate of Chryseobacterium indologenes Mariagrazia Perilli, Bibiana Caporale, Giuseppe Celenza, Cristina Pellegrini, Jean Denis Docquier, Marilina Mezzatesta, Gian Maria Rossolini, Stefania Stefani, and Gianfranco Amicosante* Dipartimento di Scienze e Tecnologie Biomediche, Università di L’Aquila, I-67100 L’...
متن کاملWhole genome sequencing for deciphering the resistome of Chryseobacterium indologenes, an emerging multidrug-resistant bacterium isolated from a cystic fibrosis patient in Marseille, France
We decipher the resistome of Chryseobacterium indologenes MARS15, an emerging multidrug-resistant clinical strain, using the whole genome sequencing strategy. The bacterium was isolated from the sputum of a hospitalized patient with cystic fibrosis in the Timone Hospital in Marseille, France. Genome sequencing was done with Illumina MiSeq using a paired-end strategy. The in silico analysis was ...
متن کامل